No replies to this topic

[rachgibson]

Hi there

I was wondering if anyone could please give me some advice, I have two  independent sets of microarray data which are Agilent one colour for gene expression.  My plan was to compare Wildtype to mutant expression profiles.  I am  having major problems as all samples, mutant and the wildtype, show a  bimodal distribution of intensity.  I was wondering if anyone else has  experienced this and has any advice?  I am new to microarrays and completely lost?  The data is not normally distributed so am unable to use conventional modelling/ bioinformatic resources like babelomics as all assume a normal distribution.

Any advice would be greatly appreciated

Thanks
Rachel