Boiling, protein aggregation......reversal?? (western)
Posted 06 May 2011 - 02:27 AM
I wanted to run some important samples on SDS PAGE today but I made this really stupid mistake of boiling my protein samples for like 20mins at 95C instead of 5mins (thats what happens when you're working under pressure). Is there anyway I can reverse the damage caused by over-boiling. Right now, theyre sitting on ice. Im looking for a proteins at about 20kDa. Just to let you know, my sample buffer had both SDS and BME!
Posted 06 May 2011 - 04:59 AM
Posted 06 May 2011 - 07:33 AM
Posted 06 May 2011 - 09:48 AM