primer with higher melting temperature
Posted 05 May 2011 - 02:44 AM
i am doing research in cancer. I bought new primers for my work.Tm for tese forwrd and reverse primers are 73.3 and 72.9 respectively. I did PCR with these. but i didnt get any product in any temperature(45-72c).pleare give some suggestions.
Posted 05 May 2011 - 03:07 AM
I always had an alternate hypothesis....
Posted 05 May 2011 - 08:10 PM
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434