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Can one use Magnesium chloride for competent cell prep.

Started by Myco Rax, May 04 2011 09:24 PM

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#1 Myco Rax

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Posted 04 May 2011 - 09:24 PM

Hi All,
Can one use Magnesium chloride instead of calcium chloride for the preparation of competent cell for the cloning purpose?
Thank you

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#2 bob1

Thelymitra pulchella

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Excellent

Posted 05 May 2011 - 06:21 PM

You need to read the original paper by Hanahan... attached.

Attached Files

Hanahan 1983.pdf 1.48MB 8855 downloads

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