Will Imidazole interfere with the integrity of protein complex?or in other words,will it disrupt the interaction between proteins?
Because I want to do a two step purification to looking for interacting partner of my protein of interest,and I want use Ni-NTA capture/imidazole elution as the first step,then apply the eluate on Strep-tactin resin for the second step(again,will imidazole interfere with the binding of tagged protein onto Strep-tactin?)
Thank you in advence,welcome for suggestions!
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