Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Is pyrosequencing necessary?


  • Please log in to reply
2 replies to this topic

#1 EHMehtyl

EHMehtyl

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 04 May 2011 - 08:45 AM

After bisulfite treatment and PCR amplification, I was planning to use the same primers for sequencing (BigDye) but I've been told I HAVE to use pyrosequencing. Maybe this is too basic, but I can't understand why?? I'm not sure my supervisor will approve an order of such expensive biotinylated primers! Thank you for your help!

#2 methylnick

methylnick

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts
4
Neutral

Posted 09 May 2011 - 07:48 PM

is it becuase you have a pyrosequencer handy to use in your lab?

There are a lot of papers publishing results from pryosequencing as it's the flavour of the month.

nick

#3 EHMehtyl

EHMehtyl

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 11 May 2011 - 06:58 AM

Well, I was told by the person who works in the facility. They have the pyrosequencer... and charge per reaction... I'm going to try to sequence with normal primers. By the way, for direct sequencing from PCR products do you design internal primers or use the same you used for the PCR? Or you clone?

Thanks!!

Elena.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.