i would like to study the effects of a gene on cell cycle. since my cell line is difficult to transfect, i used lentivirus to infect the cell line and did stable selection. i synchronized the cells with nocodzole, and i found the percentage of cells arrest at G2 phase by nocodazole between the cells infected with vector control and my gene was different, even though i had tried several time points.
i would like to know what i should do to solve this problem. is this approach correct?
people do not claim they have done synchronization in papers, and is it necessary to do synchronization??
No replies to this topic