Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cell line for glutamate receptors


  • Please log in to reply
1 reply to this topic

#1 kadamb

kadamb

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 31 May 2002 - 07:52 PM

Hi! i am looking for cell lines for glutamate receptors, i am growing neurons  which i bought from stragene but has very long doubling time of 92 hours. I am looking for cell lines which has rapid growth.How good is PCL 2 cell lines anybody have used this before please share your experinece.All suggestions are welcome.
Thanks a lot.
Kadamb

#2 cchambers

cchambers

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 12 January 2005 - 08:33 PM

Not a cell line but I used to prep mixed neuronal/glial cultures from embryonic mouse or rat brains (~E14-18) for screening compounds that protected against NMDA-induced toxicity. These cultures expressed all glutamate receptor subtypes after ~7 days in culture. Harvest embryos and isolate the cortices. Cut into pieces then titurate through a fire-polished pipette in DMEM/F-12 with N2 supplements. Strain through a mesh cell strainer and count (determine viability with trypan blue). Hope this helps.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.