Remove cells from dish and fix them in slides
Posted 04 May 2011 - 02:01 AM
I urgently need to remove cells from dishes and set them on slides for staining.
Given the limited resources would have someone help me if this results?
Remove cells from dish with trypsin-EDTA, centrifuged them and dissolve them in 1% paraformaldehyde and puts them in a normal microscopy slide (I don't have access to salinized slides) until dry.
Posted 04 May 2011 - 02:07 PM
Posted 05 May 2011 - 02:04 AM
But the cells grow in both sides of the coverslip, and as I need infect the cells, the majority of the inocullum stays bellow the coverslip and in the spaces of the well with no coverslip.
So, I would like to try infect the cells in dishes/plates and, posteriorly, try to remove them from the dish and fix them in slides to coloration.
Anyone could help me with that?
Posted 05 May 2011 - 06:29 PM