Questions about DEPC treatment
Posted 02 May 2011 - 11:25 AM
I'm confused about using DEPC treated water, it's recommended to dissolve RNA pellet with DEPC treated water, and at the same time not recommended to clean the glasswares with it, because it have bad effect on RNA, Protein and RT-PCR reaction, if so, why we dissolve the RNA pellet with DEPC treated water?
I prepared DEPC treated water before 2 months, but now i saw something like cloudy mass in the bottle , is it normal? i never saw like this before!!
Posted 02 May 2011 - 03:32 PM
Posted 03 May 2011 - 08:41 AM
depc is highly toxic so you won't want to handle it too much.
that being said, after washing the labware that we use for rna, we would treat with depc (soak the labware in water with depc then autoclave the whole mess).
Edited by mdfenko, 03 May 2011 - 08:41 AM.
genius does what it must
i do what i get paid to do