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siRNA screening 96well plate


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#1 LudosB

LudosB

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Posted 03 May 2011 - 06:13 AM

Hi all,
I'm working on a screening in 96 well plate...now I have to stain the KD cells with phalloidin and tubulin.
Do u have any idea why the phalloidin staining is so orrible!?!?!?!....we normally do the same staining on coverslips and works perfectly and when I go to a 96 well plate the result is not good at all.
By the way I use a glutaraldehyde staining protocol...
Thank guys.
Cheers
Ludos




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