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#1
VanderWall
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Posted 02 May 2011 - 08:19 PM
How can I calculate the % DNA denatured at 30 degrees after cooling from a higher strand seperation temperature such as 100 degrees as in the picture?
#2
bob1
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Thelymitra pulchella
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Posted 04 May 2011 - 01:56 PM
At 30 degrees (assuming Celsius here, not Fahrenheit) there should be very little DNA denatured - after all, in the cell, at 37 degrees C DNA is normally double stranded.
