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DNA denaturation and renaturation


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#1 VanderWall

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Posted 02 May 2011 - 08:19 PM

How can I calculate the % DNA denatured at 30 degrees after cooling from a higher strand seperation temperature such as 100 degrees as in the picture?

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#2 bob1

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Posted 04 May 2011 - 01:56 PM

At 30 degrees (assuming Celsius here, not Fahrenheit) there should be very little DNA denatured - after all, in the cell, at 37 degrees C DNA is normally double stranded.




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