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[Ambinlab]

Dear all,

I want to do CO-IP to see if two proteins are interacting with each other. One is 90kD and the other is around 50kD. What should I get if these two are interacting on the western blot?

thanks sorry I know this is a stupid question

[kfunk106]

Assuming there is no permanent linking involved (not a covalent interaction) you should get the 90 kDa protein at 90 kDa and the 50 kDa protein at 50 kDa.