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Co-IP, what should I get?


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#1 Ambinlab

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Posted 02 May 2011 - 04:53 PM

Dear all,

I want to do CO-IP to see if two proteins are interacting with each other. One is 90kD and the other is around 50kD. What should I get if these two are interacting on the western blot?

thanks sorry I know this is a stupid question

#2 knuf

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Posted 03 May 2011 - 06:11 AM

Assuming there is no permanent linking involved (not a covalent interaction) you should get the 90 kDa protein at 90 kDa and the 50 kDa protein at 50 kDa.




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