Posted 02 May 2011 - 10:45 AM
When the contamination was first noted it was at a low enough level that it wasn’t interfering with cellular proliferation and as long as the cells were washed and sub-cultured every day it was manageable. However, over the weekend the contamination has increased significantly and the cells are starting to lose their morphology. A small white speck began to grow on the top portion of the culture flask – giving me further evidence that this is most likely caused by a fungus.
I’m at a total loss as for what to do now since nothing seems to be helping clear it up. I would rather leave discarding the culture entirely and starting over as an absolute last resort since that will delay us 2-3 months but it looks like that might be the only option.
Posted 02 May 2011 - 03:34 PM
Posted 03 May 2011 - 07:31 AM
Posted 03 May 2011 - 08:27 PM
When you repeat this again, I would have a backup flask running at the same time, and I would freeze down some of the cells periodically so you don't have to go back to square one should you have contamination again.
I would probably freeze a vial every 2 weeks or so, when you are doing a split.