bacterial growth in buffers
Posted 01 May 2011 - 11:11 PM
I wanted to know if there is any chemical that can be used to prevent bacterial growth while making buffers containing salts like Tris,KCl?
I mean something that can be added such that long term use and storage is possible.Iam asking this because inspite of autoclaving i see bacterial growth in just two months time.
Instead of autoclaving after the buffer preparation i just autoclaved the glassware,water etc and prepared the same in laminar air flow chamber.Still growth has appeared.
Thanks if any of you could help:)
Posted 02 May 2011 - 03:53 AM
Posted 02 May 2011 - 03:40 PM
You could filter sterilize if you want to.