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Will transfected plasmids induce cytokine production?


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#1 Mariobro

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Posted 01 May 2011 - 08:33 AM

Hi there,

I am doing a plasmid-based shRNA knockdown experiment. I transfected plasmids and found some cytokines, such as IL6, were induced (detected via qPCR and ELISA).

That seems to be an immune reaction to me. Does anyone encounter a similar problem? Why does that happen? It would be great if you can throw some solutions. :)

Thanks a lot!

#2 pcrman

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Posted 09 May 2011 - 07:33 PM

Yes, it is possible. You can search pubmed or google to find literature on this matter.




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