I am doing a plasmid-based shRNA knockdown experiment. I transfected plasmids and found some cytokines, such as IL6, were induced (detected via qPCR and ELISA).
That seems to be an immune reaction to me. Does anyone encounter a similar problem? Why does that happen? It would be great if you can throw some solutions.
Thanks a lot!
Will transfected plasmids induce cytokine production?
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