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Salts for 6His-tag protein purification by IMAC


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#1 Yasser Almeida Hernández

Yasser Almeida Hernández

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Posted 29 April 2011 - 08:02 AM

Hi all...
I'm trying an IMAC purification of a 105 kDa glycoprotein, using copper sulfate (II). The protein is expressed by mammalian cells and is secreted to the culture medium.
I see that for this type of purification Cu, Ni, Zn and Co sulfates are the recommended salts to use depending to the selectivity/loading required for the protein and the sample.

There is another salts of this cations that could be used for the IMAC purification? Cloride salts for example?

Thanks in advance.




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