I'm trying an IMAC purification of a 105 kDa glycoprotein, using copper sulfate (II). The protein is expressed by mammalian cells and is secreted to the culture medium.
I see that for this type of purification Cu, Ni, Zn and Co sulfates are the recommended salts to use depending to the selectivity/loading required for the protein and the sample.
There is another salts of this cations that could be used for the IMAC purification? Cloride salts for example?
Thanks in advance.
Salts for 6His-tag protein purification by IMAC
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