Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

PBMC isolation from 24hr old patient blood

  • Please log in to reply
1 reply to this topic

#1 JCbio



  • Members
  • Pip
  • 1 posts

Posted 28 April 2011 - 12:31 PM

Hi, I need help.

In my lab, we have to isolate PBMC from 24hr old patient blood, then do Ab staining and FACS afterwards.
Routine procedure: 1000xg, 10min to spin down the whole blood(8-9ml), get most of the plasma to other team, then resuspend the blood with DPBS, total 14 ml. load to 2 Accuspin columns(7ml each), 800xg,15mins, no brake. The PBMCs isolated usually were heavily contaminated with Neutrophil, and not much monocyte enrichment.However,the same method always works with fresh blood and 24hr old blood from healthy subjects.
What should I do? Is it really due to the sick blood?

#2 lab rat

lab rat

    Why does a science forum not have pictures of mice and rats?

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts

Posted 28 April 2011 - 06:35 PM

Fresh blood really is better, and it could depend on what they're sick with.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.