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how to purify small fragment of DNA?


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#1 HS

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Posted 30 January 2002 - 05:36 PM

I need to purify 20mer from a mixture containing 200mer and 20mer.
How to purify this small fragment?
Thanks~~

#2 Cthulhu

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Posted 31 January 2002 - 06:08 AM

Hi,

I suggest you purify it using gel separation. You can use NuSieve GTG agarose (FMC Bioproducts). It is for separating fragments that are under 1 kb in length. You then cut out the band, and the agarose can be digested with beta-agarase.  


#3 ganeshbehere

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Posted 29 December 2004 - 07:05 PM

Hi
this is Ganesh Behere doing PhD in Genetics, I am trying to Isolate the genomic DNA from the clone saver cards with FTA the DNA spotted on the cards is nly one month old .I would really appreciate if some one asssit me in these matter.
Ganesh




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