I need a recipe for RIPA cell lysis buffer...,the one which i used is not compatible to quantify using bradford assay(SORRY I DNT HAVE OTHER OPTION TO QUANTIFY CURRENTLY),
The recipe which i used (for 100ml)...,
5ml 1M tris Hcl (ph 7.4)
3ml 5M nacl
1ml 10% NP40
0.5g sodium deoxycholate
1ml 0.5M EDTA
0.2ml 0.5M EGTA
i got the recipe from this forum, this is good but couldnt quantify using bradford assay...
Please suggest me a recipe (but stronger like RIPA) with bradfdord compatibility.....
Thanks in advance,
Edited by GNANA, 28 April 2011 - 09:20 AM.