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magnesium gtp solution


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#1 amystraydog

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Posted 28 April 2011 - 08:04 AM

I have been scouring the net looking for ways to make Mg-GTP to be used in electrophysiology experiments.  Everyone who uses it in their paper cites a paper from 1979 for the recipe, which is unaccessible.  I've read a few people mixing MgCl with GTP, but others say that the Cl causes GTP to precipitate.  Can anyone help?!

#2 phage434

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Posted 28 April 2011 - 05:00 PM

This is why we have the "paper request" subforum.  It's foolish to work without a key reference.

#3 mdfenko

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Posted 29 April 2011 - 08:23 AM

i don't think the chlorine will cause the gtp to precipitate but you can use mgso4.
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