Hi everyone,
I wanted to save some time on my protocol, and was wondering if one is able to directly PCR genomic DNA from cell lysate without first isolating the DNA. The cell in question is bacterial (salmonella). Can anyone describe the protocol and lysis buffer they used, as well as their experiences with it? Thanks in advance!
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Adrian K
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Posted 27 April 2011 - 06:02 PM
Yup, tip touch the pure colony on agar and suspend in PCR master mix. This is the fastest way.
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
