How do I determine what organic solvent to dissolve the lipid constituents in? No specific solvent is specified in the protocol. Should I start trying with the standard chloroform:methanol mix? (What exactly is the purpose of adding methanol to chloroform?)
Then a secondary question becomes, how am I supposed to determine what ratio to mix the solvents in?
Then the final dilemma becomes, how do I determine how much organic solvent is needed to dissolve the lipids? I will be using about ~5 mg total lipids. Doe it vary between lipid constituent mixtures?
Edited by research newbie, 27 April 2011 - 02:15 PM.