Designing a multiple cloning site
Posted 27 April 2011 - 09:43 AM
Posted 27 April 2011 - 11:30 AM
For details on the MCS of pBluescript (containing nearly all common REs) see this [url="[url]http://www.fermentas.com/en/support/technical-reference/phage-plasmid-dna/pbluescriptII"]link[/url][/url]!
I want to get a short genetic sequence manufactured so that I can put a gene into it. I would like to include a couple of multiple cloning sites and I was wondering whether there was a program which I could input desired enzymes and have it choose an optimal configuration of bases that would enable those enzymes to cut with a minimal number of basepairs (i.e. it would make the target sites overlap as much as physically possible)?
Posted 28 April 2011 - 12:43 AM
Posted 28 April 2011 - 03:26 AM
Posted 28 April 2011 - 04:03 AM
Posted 28 April 2011 - 06:39 AM
I would use Ape for a problem like this...