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Method to dissolve agarose gel?


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#1 Marvin

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Posted 26 April 2011 - 12:30 PM

Hi, Folks

I have some strains of fungi cultured on 2% Malt Extract agar. I want to dissolve a piece of agar with fungal hyphal in methanol, in order to test some organic compounds in the fungi (ergosterol, similar as cholesterol).

I know it can be done with some commercial kits, but don't know if there is a certain solution that can dissolve the agar.

Thanks!

#2 Adrian K

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Posted 26 April 2011 - 06:23 PM

try heat it up to 60C or to near boiling temperature in water bath?
Since is in agar, can't you possible remove it by using forceps?
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#3 phage434

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Posted 26 April 2011 - 06:30 PM

NEB sells beta-agarase, an enzyme that dissolves agar.




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