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Biotin - BMCC


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#1 red devil

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Posted 26 April 2011 - 10:19 AM

Hi,
I am performing an acyl biotin exchange with Biotin-BMCC (Pierce). I use avidin-beads from Pierce to isolate the biotinylated proteins. To get the proteins to come off of the beads, I add Non reducing buffer and boil for 7 minutes before performing Western blot. My protein of interest is only detected in non-reducing dye (Western blotting) hence I cannot use reducing dye. Is there as way around this?

red devil

#2 mdfenko

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Posted 27 April 2011 - 11:51 AM

you could use a different primary antibody (one that will recognize the reduced protein).

Edited by mdfenko, 27 April 2011 - 11:52 AM.

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