Biotin - BMCC
Posted 26 April 2011 - 10:19 AM
I am performing an acyl biotin exchange with Biotin-BMCC (Pierce). I use avidin-beads from Pierce to isolate the biotinylated proteins. To get the proteins to come off of the beads, I add Non reducing buffer and boil for 7 minutes before performing Western blot. My protein of interest is only detected in non-reducing dye (Western blotting) hence I cannot use reducing dye. Is there as way around this?
Posted 27 April 2011 - 11:51 AM
Edited by mdfenko, 27 April 2011 - 11:52 AM.
genius does what it must
i do what i get paid to do