Cloning direction / orientation?
Posted 26 April 2011 - 09:49 AM
People have been telling me to do either sequencing or restriction analysis.
The sequencing machine in my lab is very fully occupied recently, so I can't use it.
Can anyone tell me how to use restriction analysis to check for the direction of cloning?
tell me the protocol and the principle behind it because i don't really understand how this works.
is there any other ways to check it?
Posted 26 April 2011 - 08:19 PM
Posted 27 April 2011 - 03:49 AM