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a second small peak to the right to my pricipal peak!!!

Started by owen1982, Apr 26 2011 06:11 AM

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#1 owen1982

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Posted 26 April 2011 - 06:11 AM

[size="6"][size="5"]hello every body,
i'm doing my PCR tests using a stratagen MX3005P and SYBRgreen.
i'm having a second small peak at different C° whiec are higher than the C° of my desiered peak!!!!
is there any one who can suggest to me the reason why i'm having that and if yes what should i do....
THX a lot.beta actin.png

#2 Trof

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Posted 26 April 2011 - 09:06 AM

Unspecific product, try to increase stringency of the reaction, higer Ta or less magnesium.
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