Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

stripping of Western blot?


  • Please log in to reply
1 reply to this topic

#1 rick112

rick112

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 71 posts
0
Neutral

Posted 25 April 2011 - 09:31 PM

hi

could anyone let me know stripping of western blot work??

thank a lot

regards

#2 protolder

protolder

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 293 posts
9
Neutral

Posted 25 April 2011 - 09:53 PM

Hola, it releases all the bounded proteins to the membrane, blocking agent as antibodies. Moreover some of the transferred protein too, for that after stripping coulored markers loose intensity. But after stripping you could reprobing the membrane against other primary antibody .Buena suerte




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.