could anyone let me know stripping of western blot work??
thank a lot
stripping of Western blot?
1 reply to this topic
Posted 25 April 2011 - 09:53 PM
Hola, it releases all the bounded proteins to the membrane, blocking agent as antibodies. Moreover some of the transferred protein too, for that after stripping coulored markers loose intensity. But after stripping you could reprobing the membrane against other primary antibody .Buena suerte