Is it possible to blunt only either the 5' or 3' end?
Posted 24 April 2011 - 04:45 PM
Posted 26 April 2011 - 08:01 AM
Is it possible to blunt only either the 5' or 3' end of a DNA fragment or do all blunting enzymes blunt indescriminately?
I contacted NEB and they said exoT with cut 3' overhangs and RecJf will cut 5' overhangs.
In any case, neither are necessary if I cut with one enzyme and blunt that end before denaturing and cutting with the enzyme whose sticky ends I want to keep!