Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Is it possible to blunt only either the 5' or 3' end?


  • Please log in to reply
1 reply to this topic

#1 seanspotatobusiness

seanspotatobusiness

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 175 posts
7
Neutral

Posted 24 April 2011 - 04:45 PM

Is it possible to blunt only either the 5' or 3' end of a DNA fragment or do all blunting enzymes blunt indescriminately?

#2 seanspotatobusiness

seanspotatobusiness

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 175 posts
7
Neutral

Posted 26 April 2011 - 08:01 AM

Is it possible to blunt only either the 5' or 3' end of a DNA fragment or do all blunting enzymes blunt indescriminately?


I contacted NEB and they said exoT with cut 3' overhangs and RecJf will cut 5' overhangs.

In any case, neither are necessary if I cut with one enzyme and blunt that end before denaturing and cutting with the enzyme whose sticky ends I want to keep!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.