Is it possible to blunt only either the 5' or 3' end of a DNA fragment or do all blunting enzymes blunt indescriminately?
0
Is it possible to blunt only either the 5' or 3' end?
Started by seanspotatobusiness, Apr 24 2011 04:45 PM
1 reply to this topic
#2
seanspotatobusiness
-
- Active Members
-
- 115 posts
Veteran
1
Neutral
Neutral
Posted 26 April 2011 - 08:01 AM
seanspotatobusiness, on 24 April 2011 - 04:45 PM, said:
Is it possible to blunt only either the 5' or 3' end of a DNA fragment or do all blunting enzymes blunt indescriminately?
I contacted NEB and they said exoT with cut 3' overhangs and RecJf will cut 5' overhangs.
In any case, neither are necessary if I cut with one enzyme and blunt that end before denaturing and cutting with the enzyme whose sticky ends I want to keep!
