Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Imaging western blot membrane


  • Please log in to reply
2 replies to this topic

#1 uvbox

uvbox

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 22 April 2011 - 06:36 PM

Hi all,

Can you leave western blot membranes in TBST after secondary IgG has been applied and image them within the next two days (instead of straight after?
Will this affect the expression of my protein at all?

Ta! :)

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,757 posts
130
Excellent

Posted 25 April 2011 - 07:01 AM

you may be able to. i would leave the tween out.
talent does what it can
genius does what it must
i do what i get paid to do

#3 uvbox

uvbox

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 01 June 2011 - 09:22 PM

Left it over the weekend, imaged it on monday and turned out fine :)





you may be able to. i would leave the tween out.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.