Can you leave western blot membranes in TBST after secondary IgG has been applied and image them within the next two days (instead of straight after?
Will this affect the expression of my protein at all?
Ta!
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2 replies to this topic
#1
uvbox
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Posted 22 April 2011 - 06:36 PM
Hi all,
Can you leave western blot membranes in TBST after secondary IgG has been applied and image them within the next two days (instead of straight after?
Will this affect the expression of my protein at all?
Ta!
Can you leave western blot membranes in TBST after secondary IgG has been applied and image them within the next two days (instead of straight after?
Will this affect the expression of my protein at all?
Ta!
#2
mdfenko
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Posted 25 April 2011 - 07:01 AM
you may be able to. i would leave the tween out.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
uvbox
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Posted 01 June 2011 - 09:22 PM
Left it over the weekend, imaged it on monday and turned out fine
mdfenko, on 25 April 2011 - 07:01 AM, said:
you may be able to. i would leave the tween out.
