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RT-PCR t-test, and ANOVA


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#1 NicoBxl

NicoBxl

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Posted 21 April 2011 - 11:44 PM

Hi,

Can you help me to analyze my RT-PCR data. I've the CT for all my samples and I've compute the dCT. I've four groups of samples ( one group per condition) and I want to perform ANOVA. How can I do that ? in R ?

Here's a example of my dCT matrix
        ctrl1  ctrl2  ctrl3     A1     A2     A3     B1     B2     B3
gene1   7.307  0.237  0.611  9.640  8.705  8.870  7.307  0.611  0.237
gene2   9.061 10.111 11.016 12.025 11.482 10.982  9.061 11.016 10.111
gene3   7.903 12.048 11.911 10.796 10.931 10.648  7.903 11.911 12.048
gene4  11.731 17.809 15.951 19.682 16.964 14.036 11.731 15.951 17.809
gene5   9.464  8.955  9.351 11.730 10.652 11.009  9.464  9.351  8.955
gene6   5.737  6.539  7.557  9.279  8.057  8.115  5.737  7.557  6.539
gene7  12.971 10.800 11.314 14.235 13.043 12.999 12.971 11.314 10.800
gene8   6.756  6.060  6.297  8.254  7.907  7.680  6.756  6.297  6.060
gene9  20.093 21.098 21.488 23.132 22.913 23.040 20.093 21.488 21.098
gene10 11.943 13.582 14.404 15.425 15.627 13.367 11.943 14.404 13.582

Thanks,

N.

#2 NicoBxl

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Posted 26 April 2011 - 06:28 AM

I read that the Wilcoxon test is better than the t-test for this kind of data . Is it true ?




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