a high background of western
Posted 21 April 2011 - 11:25 PM
Posted 22 April 2011 - 06:40 AM
Posted 04 May 2011 - 05:27 AM
Try blocking with 5% non-fat milk in PBS. Also dilute your antibodies in this. Also I've found that if you use your primary at too high a concentration you can get a lot of background. And wash LOADS! With plenty of wash buffer.