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DNA in Lipofectamine 2000 Transfections


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#1 mutant11

mutant11

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Posted 21 April 2011 - 01:02 AM

I need to do some mammalian cell culture transfections and had 2 quick questions. I know that transfections depend a lot on different cell types (MCF10A, MDCK, and MCF7a) but in general what has your experience been with this. BTW, I am using lipofectamine 2000. :

Are DNA plasmids purified from Qiagen-maxiprep kits (the non-endotoxin free version) generally pure enough to use for transfections?

Can I use DNA resuspended in TE buffer (.5 mM EDTA) for transfections? I read in current protocols that DNA in TE buffer with greater than .3 mM EDTA could interfere with lipofectamine transfection.

Thanks!




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