2 replies to this topic

[Boba]

I am overexpressing AKT1 in normal breast cells and I am seeing weird things when cells are treated in full growth conditions understeady state: in one cell line there is an increase in phosporylation of AKT and in the other cell line there is no change. Can someone help explain this. I expect an inhibition.
thanks

[castleinthesky]

This may be caused by the compensation effects of other signaling pathway, you chould use other AKT inhibitors instead.

[Inmost sun]

so, the molecular environment (expression of proteins and their regulation)  for AKT1 is different in your cell lines