Submit your paper to J Biol Methods today!
help with ly294002 results
2 replies to this topic
Posted 20 April 2011 - 07:59 PM
I am overexpressing AKT1 in normal breast cells and I am seeing weird things when cells are treated in full growth conditions understeady state: in one cell line there is an increase in phosporylation of AKT and in the other cell line there is no change. Can someone help explain this. I expect an inhibition.
Posted 02 March 2012 - 07:44 AM
so, the molecular environment (expression of proteins and their regulation) for AKT1 is different in your cell lines