Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

help with ly294002 results


  • Please log in to reply
2 replies to this topic

#1 Boba

Boba

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 20 April 2011 - 07:59 PM

I am overexpressing AKT1 in normal breast cells and I am seeing weird things when cells are treated in full growth conditions understeady state: in one cell line there is an increase in phosporylation of AKT and in the other cell line there is no change. Can someone help explain this. I expect an inhibition.
thanks

#2 castleinthesky

castleinthesky

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 24 February 2012 - 12:40 AM

This may be caused by the compensation effects of other signaling pathway, you chould use other AKT inhibitors instead.

#3 Inmost sun

Inmost sun

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 303 posts
60
Excellent

Posted 02 March 2012 - 07:44 AM

so, the molecular environment (expression of proteins and their regulation) for AKT1 is different in your cell lines




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.