Sequencing of 18srRNA
Posted 20 April 2011 - 09:50 AM
It would be grateful if someone could help me out to come through these problems.
Posted 20 April 2011 - 08:51 PM
If (1) not sure about how to fix this but if (2) you might want to try changing your thrermocycling parametres (i.e. increase annealing temperature or doing a touchdown PCR) that is if it is the first PCR that is causing the problem (which it might not be). Otherwise there is another thread discussing called "Cycle sequencing (Big Dye)" that began recently, that might be helpful if the probelm is being caused by your sequencing reaction causing poor reads.
Posted 21 April 2011 - 10:43 AM
see these attachments
genius does what it must
i do what i get paid to do