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why no plastic dish for confocal?


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#1 davince

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Posted 20 April 2011 - 09:38 AM

Hello everyone.
I can access a Leica SP5 confocal microscope.
i have a question.
i know most of the time we are using glass slide and glass coverslip.
the thing is i don't know the reason why we can't use plastic dish for confocal microscope?

i thought laser from confocal microscope comes in and out in objectives, and suppose i'm using a coverglass. then fluorescence from the labelling of the cells should still be captured?

can anyone tell me why we can't use polystyrene dish for confocal microscope?


thank you

#2 Rsm

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Posted 21 April 2011 - 01:31 AM

Most of the times it is either too thick or has a wrong refraction index.
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#3 bob1

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Posted 26 April 2011 - 08:24 PM

They are also very uneven, so at high magnifications you will get odd diffraction patterns from the changes in the surface.




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