2 replies to this topic

[davince]

Hello everyone.
I can access a Leica SP5 confocal microscope.
i have a question.
i know most of the time we are using glass slide and glass coverslip.
the thing is i don't know the reason why we can't use plastic dish for confocal microscope?

i thought laser from confocal microscope comes in and out in objectives, and suppose i'm using a coverglass. then fluorescence from the labelling of the cells should still be captured?

can anyone tell me why we can't use polystyrene dish for confocal microscope?


thank you

[Rsm]

Most of the times it is either too thick or has a wrong refraction index.

[bob1]

They are also very uneven, so at high magnifications you will get odd diffraction patterns from the changes in the surface.