Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

why no plastic dish for confocal?

  • Please log in to reply
2 replies to this topic

#1 davince



  • Members
  • Pip
  • 3 posts

Posted 20 April 2011 - 09:38 AM

Hello everyone.
I can access a Leica SP5 confocal microscope.
i have a question.
i know most of the time we are using glass slide and glass coverslip.
the thing is i don't know the reason why we can't use plastic dish for confocal microscope?

i thought laser from confocal microscope comes in and out in objectives, and suppose i'm using a coverglass. then fluorescence from the labelling of the cells should still be captured?

can anyone tell me why we can't use polystyrene dish for confocal microscope?

thank you

#2 Rsm


    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 385 posts

Posted 21 April 2011 - 01:31 AM

Most of the times it is either too thick or has a wrong refraction index.
I got soul, but I'm not a soldier

#3 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,511 posts

Posted 26 April 2011 - 08:24 PM

They are also very uneven, so at high magnifications you will get odd diffraction patterns from the changes in the surface.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.