stable transfection of epithelial cells
Posted 09 June 2002 - 12:15 PM
Posted 11 June 2002 - 03:52 AM
Posted 12 June 2002 - 06:34 AM
So I think the problem lies in both transfection itself where i do not get plasmid integration and also in selection.
I allow 48 hours of selection after transfection as this is the time taken for the control cells to die after I add puromycin.This is what I got when I did the death response experiment.
I use a CMV promoter driven plasmid. I wonder if CMV promoter is good for creating stable cell lines for the ones I am using.
Posted 12 June 2002 - 06:46 AM
Posted 12 June 2002 - 10:39 AM
Posted 12 June 2002 - 11:32 AM
Posted 13 June 2002 - 12:08 PM
Posted 13 June 2002 - 02:20 PM
I am also trying to find if CMV vectors are good systems for epithelial cells but have not been able to find anything conclusive, though some references do seem to be using CMV driven systems.