I would like to ask if 16% PFA was diluted in water instead of PBS and used for fixation,what would happen to the cells?
thanks
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#1
hrltwc
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Posted 19 April 2011 - 12:00 PM
Hi,
I would like to ask if 16% PFA was diluted in water instead of PBS and used for fixation,what would happen to the cells?
thanks
I would like to ask if 16% PFA was diluted in water instead of PBS and used for fixation,what would happen to the cells?
thanks
#2
bob1
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Thelymitra pulchella
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Posted 19 April 2011 - 03:28 PM
They would be quite unhappy - this would cause an increase in osmotic pressure on the cells, causing them to take up water and probably burst.
