I don't really understand what is the nature of HEPES (acid or base ?).
What is the difference between HEPES (free acid) and HEPES sodium salt...?
What will be the difference in adding HEPES between 2 samples, one with pH=7.5 and the other with pH=8.1.
Should i for each one prepare a HEPES solution specifically for these two samples? i mean should i before adding HEPES in my solution prepare 2 HEPES solutions with the 2 differents pH.
Same question: Must HEPES solutions have the same pH that there is in my samples ?
The aim of my experiment is to stabilish pH in 2 flasks where the pH of my seawater has been decreased with HCl to 7.5 and to 8.1(No HCl in this one it's ambiant seawater)
I hope i have been clear, sorry for my english...
Hope you could help me
Edited by polo24, 19 April 2011 - 11:56 AM.