My neuroblastoma are floating!! please help
Posted 19 April 2011 - 09:34 AM
I have cultured the SK-N-SH and the SH-SY5Y cell lines for many years in grad. school. I never had any trouble when thawing them, but now in a new lab they stay floating. I have centrifuged it after being 24 hours in the incubator but it has not attached. I am doing all the same i used to do before, but can't figure out why cells are not attaching to the flask. I already tried changing the size and bran of the flask but the same happens. What other posibilities might be causing this?
Posted 19 April 2011 - 03:25 PM
Sh-SY5Y have a mixed population of cells with some being adherent and some floating, both should be cultured!
Posted 19 April 2011 - 03:34 PM
Posted 20 April 2011 - 09:31 AM
then heaven will be yours, before you meet your end