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Phalloidin on Human Brain


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#1 cassi

cassi

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Posted 18 April 2011 - 02:41 PM

How do I get phalloidin to work on Human Brain sections? I have brain sections that are acetone fixed and para-formaldehyde fixed and it won't work on either. But I can get it to work on cell lines so I know it is not a problem with the phalloidin or the microscope. I do no use methanol and I do permeate the cells with triton x 100, block with 10% goat and add the phalloidin for 2 hours and still nothing. Has anyone ever stained human brain with phalloidin and if so how did you do it?




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