One Shot TOP10 Cells
Posted 18 April 2011 - 12:25 AM
I have discovered a box of One Shot TOP10 competent cells deep within our deep freezer. So I am very curious about how I can use this.
I have looked up a manual for these cells and it says that "TOP 10 Cells do not require IPTG to induce expression from the Lac promoter".
So my question is- if I have a plasmid from the pET system under T7 promoter control, I still need to add IPTG to induce my target protein right??
I am also looking at the genotype for this. What does StrR mean? streptomycin resistance?
Posted 18 April 2011 - 02:14 AM
Edited by protolder, 18 April 2011 - 02:40 AM.
Posted 18 April 2011 - 02:39 PM
Thank you for your reply. Has anyone had experience with using this particular strain for expression?
Would it be useful for that purpose, or it it more just a cloning strain for making plasmids?
Posted 18 April 2011 - 06:43 PM
Posted 18 April 2011 - 08:56 PM
Posted 18 April 2011 - 10:42 PM
Thanks for this.