is it possible to use trypan blue to stain chlorella? will the green pigment hinder the visibility of trypan blue uptake?
I am not sure if dead cells in the stationary phase retain chlorophyll pigment or not.
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trypan blue for chlorella
Started by belladonna22, Apr 17 2011 08:15 AM
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belladonna22
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Posted 17 April 2011 - 08:15 AM
Hi
is it possible to use trypan blue to stain chlorella? will the green pigment hinder the visibility of trypan blue uptake?
I am not sure if dead cells in the stationary phase retain chlorophyll pigment or not.
is it possible to use trypan blue to stain chlorella? will the green pigment hinder the visibility of trypan blue uptake?
I am not sure if dead cells in the stationary phase retain chlorophyll pigment or not.
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kajmak
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Posted 24 April 2011 - 03:48 PM
Eventually chlorophyll ‘disappears’ but it should not be a problem since trypan blue is very violent stain. Can you use some other stain? Trypan blue is not nice to work with. It is toxic to cells and sometimes it is a cause of cell death. Propidium iodide works on the same principle and is nice and easy to work with.
Edited by kajmak, 24 April 2011 - 03:49 PM.
