we are using the Qiagen Dneasy kits to isolate DNA from mouse colonic samples. however, it is the gram positive microbiota we actually want to get DNA from. The Qiagen booklet gives protocols for isolating DNA from tissue and isolating DNA from a gram positive bacterial culture, but nothing on tissue-associated gram positive bacteria understandably.
If anyone is familiar with this, my question is after homogenizing the tissue in PBS, when should the lysozyme step be performed, and should it be done in place of any of the tissue DNA isolation steps? I just don't know how to combine these two protocols.
I'd just call Qiagen tomorrow, but I'd love to be able to do this today and they're closed.
DNA Extraction from Tissue Associated Bacteria
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