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Help on BmN4 cell culture


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#1 elsalex

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Posted 16 April 2011 - 08:29 AM

Dear all,

I am looking to maintain a culture of Bombyx mori BmN4 cells.
Could anyone please give me some advice on passsage frequency and split ratios?
Also, would anyone be able to tell me how healthy BmN4 cells typically look like when attached to a surface (i.e. round or elongated, etc.)?

Thank you so much in advance!

Best,
Elsa

#2 protolder

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Posted 18 April 2011 - 12:07 AM

Dear all,

I am looking to maintain a culture of Bombyx mori BmN4 cells.
Could anyone please give me some advice on passsage frequency and split ratios?
Also, would anyone be able to tell me how healthy BmN4 cells typically look like when attached to a surface (i.e. round or elongated, etc.)?

Thank you so much in advance!

Best,
Elsa

Hola Elsa, I suposse that you want growing this line to produce recombinant proteins infecting with some baculovirus. I never have worked with this line, but in general insect cells grow in monolayer and suspension the split ratio could be (as the manuals say ) 1/8 each 3-4 days from an 80-90% confluence. Sf9 ,Tn5 are round but ask to him give them you or look the data sheet if you buy them.Or wait the answer of anybody that known they Buena suerte

Edited by protolder, 18 April 2011 - 12:08 AM.





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