I am testing a couple of anti-angiogenic compounds using "capillary-like tube formation assay". I use HUVEC cells and matrigel to do this. I have observed that the cells form tubes within 2 hrs, but after overnight, most cells die and only a clumps of cells are seen to be scattered randomly. This phenomenon is boosted by the inclusion of serum in the growth media. My question is 1) is it normal to see this phenomena and 2) If not, how do I get around this problem.
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