Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Compare ChIP to cell lysate

  • Please log in to reply
1 reply to this topic

#1 mcb56



  • Active Members
  • Pip
  • 29 posts

Posted 15 April 2011 - 06:24 PM

I am doing a chromatin immunoprecipitation and I would like to compare the amount of protein I purify with my antibody to the amount of the same protein left over in my cell lysate. I can't use a loading control because the ChIP will only have my protein of interest and nothing else to compare it to.

Thank You

#2 chabraha



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 137 posts

Posted 11 May 2011 - 07:54 AM

you should just be able to do a regular old IP and compare your Input (either 1 or 10 percent) to what you pull down with your beads.......after washing the beads hit it with sample buffer, boil for 10min and run it on your gel. Doing that should get you a ballpark figure for how well your antibody IP's in cross-linked conditions.
Treasure Chest Wizardry

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.