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Strange vesicles seen post GFP transfection


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#1 blackhall

blackhall

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Posted 15 April 2011 - 06:33 AM

Hi guys.

I have transfected some immortalised synoviocytes with GFP to assess transfecton efficiency.
However, when visualised, there appears to be fluorescent, granular vesicles intracellularly.
the samples were visualised 24 hours post transfection.

Could these be protein synthesis compartments?? Anyone every seen this.

Thanks,

Blackhall




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